Their rather intimate looking kiss made worldwide headlines following the 2000 Oscar ceremony.
So it was hardly surprising then, that Angelina Jolie and her older brother James Haven kept their distance on the red carpet last night.
James, 38, was showing his support to Angelina at the New York film premiere for movie In the Land of Blood and Honey, which she wrote and directed.
Brotherly love: James Haven arrived with girlfriend Ashley Reign at the New York premiere if In The Land of Blood and Honey yesterday night
But James, who arrived with his girlfriend Ashley Reign, did not pose with his sister on the carpet.
He did, however, match her all-black costume theme, wearing a dark suit and back tie.
Angelina arrived in a simple black top and long skirt with chunky necklace while her long term partner Brad Pitt wore a three-piece suit.
Separate arrivals: Angelina Jolie and Brad Pitt walked the carpet together at the event
Likeness: Just two years older, James clearly resembles his famous sister
With their razor sharp cheekbones and bee-stung lips, brother and sister clearly bore a resemblance to each other.
James and Angelina, children of actor Jon Voight and Marcheline Bertrand have always been close.
During her Oscar acceptance speech back in 2000, Angelina famously said she is ‘just so in love with my brother right now.’
Controversial: Angelina and brother James were pictured in a clinch after her Oscar win for Girl, Interrupted in 2000
As they were: Angelina pictured as a teenager with her father Jon Voight and brother James at a movie premiere
Later, at the Vanity Fair Oscar party, she and James were pH๏τographed sharing a kiss on the lips, which attracted worldwide publicity and subsequent incest jokes, which Angelina called ‘pathetic.’
‘First, we’re the best of friends. And it wasn’t some odd open-mouthed kiss. It was disappointing that something so beautiful and pure could be turned into a circus,’ she later told People.
James added: ‘It was an amazing moment. Yet it was totally misconstrued.’
Back on the trail: Angelina was back on the promotional trail today as she attended a special screening of her film in New York
Sophisticated: For the event, which was sponsored by Marie Claire, she slipped into a stylish black velvet suit and matching black silk blouse
He also told USA Today that gossip mongers ‘will realise in time that it is just a very close relationship, and it has nothing to do with what they are implying.’
Angelina was back on the promotional trail today as she attended a special screening of her film in New York.
For the event, which was sponsored by Marie Claire, she slipped into a stylish black velvet suit and matching black silk blouse.
Cast sH๏τ: Angelina poses with the cast of In The Land Of Blood and Honey
She accessorised the look with some simple black platform shoes and some gold earrings and bracelet.
Before the event she went for a spot of dinner with her entourage.
In the Land of Blood and Honey is released in the U.S. on December 23.